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Iron Acquisition from Natural Organic Matter by an Aerobic Pseudomonas mendocina Bacterium: Siderophores and Cellular Iron Status
Authors:Keshia M. Kuhn  Carolyn A. Dehner  Jennifer L. Dubois  Patricia A. Maurice
Affiliation:1. Department of Civil Engineering and Geological Sciences , University of Notre Dame , Notre Dame , Indiana , USA;2. Department of Chemistry and Biochemistry , University of Notre Dame , Notre Dame , Indiana , USA
Abstract:This research investigated the potential role of siderophores in aerobic microbial Fe acquisition from natural organic matter (NOM; XAD-8 isolate and reverse osmosis concentrate pre- and post-Chelex® treatment) through the use of a siderophore-producing Pseudomonas mendocina wild type (WT) bacterium and an engineered mutant (Mt) that was incapable of siderophore production. NOM had complex effects on microbial growth under Fe-limited conditions as measured by optical density, most likely because of the presence of other toxic (trace) metals such as Al, NOM binding interference with additional trace metal nutrients, and/or biofilm development. However, a bioassay for cellular Fe status showed that both WT and Mt readily acquired Fe naturally associated with NOM. Thus, while siderophores may be useful for Fe acquisition from NOM by P. mendocina, they do not appear to be essential for this process.
Keywords:Pseudomonas   natural organic matter   siderophores   aerobe   iron acquisition
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