Biodegradation of Aliphatic and Aromatic Hydrocarbons by Nocardia sp. H17-1 |
| |
Authors: | Kyung-Hwa Baek Byung-Dae Yoon Hee-Mock Oh Hee-Sik Kim In-Sook Lee |
| |
Institution: | 1. Environmental Biotechnology Laboratory , Korea Research Institute of Bioscience and Biotechnology , Daejeon , 305-333 , Korea;2. Department of Biological Science , Ewha Womans University , Seoul , 120-750 , Korea |
| |
Abstract: | We investigated the biodegradation of hydrocarbon components by Nocardia sp. H17-1 and the catabolic genes involved in the degradation pathways of both aliphatic and aromatic hydrocarbons. After 6 days of incubation, the aliphatic and aromatic fractions separated from Arabian light oil were degraded 99.0 ± 0.1% and 23.8 ± 0.8%, respectively. Detection of the catabolic genes involved in the hydrocarbon degradation indicated that H17-1 possessed the alkB genes for n-alkane biodegradation and catA gene for catechol 1,2-dioxygenase. However, H17-1 had neither the C23O gene for the degradation of aromatic hydrocarbons nor the catechol 2,3-dioxygenase activity. The investigation of the genes involved in the biodegradation of hydrocarbons supported the low degradation activity of H17-1 on the aromatic fractions. |
| |
Keywords: | aliphatic and aromatic hydrocarbon biodegradation catabolic genes crude oil Nocardia sp |
|
|