The Archaeal Diversity and Population in a Drained Alkaline Saline Soil of the Former Lake Texcoco (Mexico) |
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| Authors: | César Valenzuela-Encinas Rocio J. Alcántara-Hernández Isabel Estrada-Alvarado Francisco Javier Zavala-Díaz de la Serna Luc Dendooven Rodolfo Marsch |
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| Affiliation: | 1. Laboratory of Soil Ecology, Cinvestav, Mexico City , Distrito Federal, Mexico;2. Departamento de Biotecnología y Ciencias Alimentarias , Instituto Tecnológico de Sonora , Sonora, Mexico |
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| Abstract: | Draining soil of the former Lake Texcoco, Mexico with pH > 10.0 and electrolytic conductivity (EC) > 100 dS m?1 for 17 years has reduced pH to 7.8 and EC to 0.68 dS m?1. Metagenomic DNA from the archaeal community was extracted directly from this soil and used as template to amplify the 16S ribosomal genes by PCR to construct gene libraries. Most of the cloned Archaea were related to mesophilic crenarchaeota and were not-yet-cultured. Sequence and phylogenetic analyses of these clones identified a group of Archaea with close affiliation to the ammonia-oxidizing Archaea. The cloned sequences from the drained soil diverged clearly from Haloarchaea found in the undrained soil from the lake. |
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| Keywords: | 16S ribosomal genes Archaea drained soil phylogenetic analysis |
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