Biomicrospheres Generate Ooids in the Laboratory

Precipitation of calcium carbonate is widespread in microbial communities (biofilms, microbial mats, etc.) and plays an important role in the nucleation and formation of stromatolites, onkolites and oolites. Here we report carbonate precipitation under laboratory conditions in defined spherical microbial communities. These microbial communities represent a symbiosis of cyanobacteria, diatoms and heterotrophic bacteria. Calcification structures always reflect the shape of microbial aggregates resulting from the formation of coalescent grains in chains. Position of the single carbonate crystals follows the spherical arrangement of the filamentous cyanobacteria and results in hollow spheres. The structure of the spherical biofilm determines the form of the carbonate layer. Tiny calcite scleres, deposited by spherical microbial communities in concentric arrangement are observed continuously, documented and described in the laboratory cultures. They may represent the precursors of oolites and are the first reproducible laboratory system generating ooids without a hard nucleus within an otherwise laminated microbial mat community.