Toward a controllable headspace composition—Growth,development, and headspace of a micropropagatedPrunus rootstock in different containers |
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Authors: | J J Demeester D G Matthijs B Pascat P C Debergh |
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Institution: | (1) Lab. voor Tuinbouwplantenteelt, Coupure links 653, 9000 Gent, Belgium;(2) Laboratory for Horticulture, University Gent, Coupure links 653, B-9000 Gent, Belgium;(3) ADRIAC, Pôle Technologique Henri Farman, Esplanade Roland Garros, 51100, Reims Cedex, France |
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Abstract: | The body and closure device of a tissue culture container are of paramount importance in the determination of the headspace
composition, and influence plant growth and development. In fact, the container determines gas exchange with the environment
quantitatively and qualitatively. When exchange occurred via permeability, we demonstrated that a properly controlled container
atmosphere (CA) cannot be obtained because accumulation and/or depletion of gases occur; modified atmosphere (MA) is then
a better term. Our experiments also gave information about a) gas exchange mechanisms and b) the influence of headspace composition
on plant growth and development. These are prerequisites for the understanding and final control of the headspace composition.
Three containers, differing in their gas exchange mechanism, were evaluated. This paper also considered that even under “controlled”
culture conditions (container, environment, explant weight, etc.) variations from container to container are registered, probably
due to the explant itself. |
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Keywords: | headspace tissue culture container gas exchange |
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