Low temperature, gibberellin and acid lipase activity in removal of apple seed dormancy |
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Authors: | BO ENA ARSKA-MACIEJEWSKA,IRENA SI SKA,EWA WITKOWSKA,STANIS AW LEWAK |
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Affiliation: | Institute of Botany, The University of Warszawa, Poland |
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Abstract: | The activity of acid lipase and the level of gibberellin A4 (GA4) were determined in apple embryos excised from seeds after different time periods of stratification and subsequently cultured in darkness at 4°C or at 25°C. Enzyme activity and GA4 content were higher at 4°C. Exogenous gibberellin stimulated lipase activity, while AMO-1618, an inhibitor of gibberellin biosynthesis, inhibited, to the same degree, both the enzyme activity and the GA4 accumulation. The involvement of GA4 and lipolytic enzymes in cold-mediated removal of embryonal dormancy has been discussed and compared with the role of these two factors in light-stimulated germination of dormant apple embryos, described earlier (Smoleńska and Lewak 1974). |
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Keywords: | Gibberellin lipases cold apple seeds |
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