Base composition of DNA from symbiotic dinoflagellates: a tool for phylogenetic classification |
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Authors: | Rudolf J Blank Volker A R Huss Walter Kersten |
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Institution: | (1) Institut für Botanik und Pharmazeutische Biologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, D-8520 Erlangen, Germany;(2) Institut für Biochemie der Medizinischen Fakultät, Universität Erlangen-Nürnberg, Fahrstrasse 17, D-8520 Erlangen, Germany |
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Abstract: | DNA of eight endosymbiotic dinoflagellates (zooxanthellae) from seven different host species has been analyzed as to its thermal characteristics and base composition by means of spectrophotometry and high performance liquid chromatography. All algae under investigation contain both methylcytosine and hydroxymethyluracil in addition to the bases typical of nuclear DNA. As a result, melting temperatures are decreased, suggesting lower contents of guanine plus cytosine than actually present. True percentages of guanine plus cytosine plus methylcytosine range from about 43 to 54 mol%. They are unique for the symbionts from different hosts, indicating phylogenetic separation of the taxa comparised within the genus Symbiodinium.Abbreviations dA
deoxyadenosine
- dC
deoxycytidine
- dG
deoxyguanosine
- dT
deoxythymidine
- m5dC
5-methyldeoxycytidine
- hmdU
5-hydroxymethyldeoxyuridine
- rC
ribocytidine
- Br8G
bromine-80guanosine
- A
adenine
- C
cytosine
- G
guanine
- T
thymine
- m5C
5-methylcytosine
- hmU
5-hydroxymethyluracil
- G+C
guanine plus cytosine plus 5-methylcytosine
- HPLC
high performance liquid chromatography
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T
m
temperature at the midpoint of hyperchromic shift
- CTAB
N-cetyl-N,N,N-trimethyl-ammonium bromide
- EDTA
ethylenediamine-tetraacetic acid, disodium salt
- TRIS
tris-(hydroxymethyl)-aminomethane
- 1×SSC
standard saline citrate (0.15 M NaCl+0.015 M trisodium citrate, pH 7.0) |
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Keywords: | Dinoflagellates DNA composition Hydroxymethyluracil Methylcytosine Speciation Symbiodinium Symbiosis Zooxanthellae |
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