| RAPD标记鉴定水稻3种不同细胞质雄性不育类型的杂交种及其亲本 |
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| 引用本文： | 徐鑫,刘学群,瞿波,朱英国,王春台. RAPD标记鉴定水稻3种不同细胞质雄性不育类型的杂交种及其亲本[J]. 遗传, 2005, 27(3): 377-381 |
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| 作者姓名： | 徐鑫 刘学群 瞿波 朱英国 王春台 |
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| 作者单位： | 1.中南民族大学生物技术国家民委重点实验室，武汉 430074; 2.武汉大学植物发育教育部重点实验室，武汉 430072; 3.华中农业大学植物科学技术学院，武汉 430070 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，湖北省科研项目 |
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| 摘 要： | 利用RAPD引物对3种不同细胞质雄性不育类型的杂交水稻组合及其亲本共21个材料进行了DNA多态性分析。从264个随机引物中筛选出具有非常明显多态性的引物25个, 对25个引物在3种不同细胞质不育类型的杂交组合及其亲本间的DNA扩增多态性差异进行比较，最终选出具有不同类型间特异性扩增带的引物7个，利用这些特异性扩增带能有效地区分和鉴定目前在生产上大面积种植和推广，或者是具有应用潜力的3种不同细胞质雄性不育类型—野败型(WA)、红莲型(HL)和包台型(BT))—的6种杂交水稻组合及其亲本。
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| 关 键 词： | 多态性 细胞质雄性不育 杂交水稻 RAPD |
| 文章编号： | 0253-9772(2005)03-0377-05 |
| 收稿时间： | 2004-06-14 |
| 修稿时间： | 2004-06-14 |
RAPD Identification of Three Types of Three-lines Hybrid Rice with Different Male Sterile Cytoplasms and Their Parent Lines |
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| XU Xin,LIU Xue-Qun,QU Bo,ZHU Ying-Guo,WANG Chun-Tai. RAPD Identification of Three Types of Three-lines Hybrid Rice with Different Male Sterile Cytoplasms and Their Parent Lines[J]. Hereditas, 2005, 27(3): 377-381 |
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| Authors: | XU Xin LIU Xue-Qun QU Bo ZHU Ying-Guo WANG Chun-Tai |
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| Affiliation: | 1. Biotechnological Laboratory of National Commission for Nationalities, College of Chemistry and Life Science, South–central University for Nationalities, Wuhan 430074,China; 2. Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan 430072 China; 3.College of Plant Science and Technology, Huazhong Agricultural University，Wuhan 430074 China |
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| Abstract: | The RAPD analysis was conducted on genome DNA from 21 sets of rice, including 6 three-line hybrid rice combinations, separately derived from three different kinds of cytoplasmic male sterile (CMS) lines and their related parents. Out of 264 random primers screened first, 25 primers displayed well in polymorphisms. It was shown that only 7 bands amplified respectively from 7 primers were enough to discriminate the different types of CMS, the hybrid combinations and their parents. |
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| Keywords: | RAPD |
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