Simultaneous analysis of several non-steroidal anti-inflammatory drugs in human urine by high-performance liquid chromatography with normal solid-phase extraction |
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Authors: | Toshio Hirai Shozo Matsumoto Ikuo Kishi |
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Affiliation: | Medical Research Laboratories, Lederle (Japan) Ltd., 1-6-34 Kashiwa-cho, Shiki-shi, Saitama 353, Japan |
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Abstract: | A practical and reproducible high-performance liquid chromatographic method using normal solid-phase extraction has been developed for the simultaneous analysis of twelve non-steroidal anti-inflammatory drugs (NSAIDs) in human urine. A urine specimen mixed with acetate buffer pH 5.0 was purified by solid-phase extraction on a Sep-Pak Silica cartridge. The analyte was chromatographed by a reversed-phase Inertsil ODS-2 column using a phosphate buffer-acetonitrile at pH 5.0 as the mobile phase, and the effluent from the column was monitored at 230 or 320 nm. Absolute recoveries were greater than 73% for all of the twelve NSAIDs. The present method enabled simple manipulation and isocratic HPLC with UV analysis as well as high sensivity of 0.005 μg/ml for naproxen, and 0.05 μg/ml for sulindac, piroxicam, loxoprofen, ketoprofen, felbinac, fenbufen, flurbiprofen, diclofenac, ibuprofen and mefanamic acid as the quantitation limit in human urine using indomethacin as an internal standard. |
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Keywords: | Naproxen Indomethacin Sulindac Piroxicam Loxoprofen Ketoprofen Fenbufen Flurbiprofen Diclofenac Iburprofen Mefenamic acid |
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