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Expression of soluble recombinant proteins in a cell-free system using a 96-well format
Authors:Busso Didier  Kim Rosalind  Kim Sung-Hou
Affiliation:Department of Chemistry, University of California, Berkeley, 314 Calvin Lab MS5230, CA 94720-5230, USA. djbusso@lbl.gov
Abstract:For structural and functional genomics programs, new high-throughput methods to obtain well-expressing and highly soluble proteins are essential. Here, we describe a rapid procedure to express recombinant proteins in an Escherichia coli cell-free system using a 96-well format. The identification of soluble proteins is performed by the Dot Blot procedure using an anti-His tag antibody. The applications and the automation of this method are described.
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