Solubilization of gellan gels by chelation of cations |
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Authors: | Landis W. Doner,Guillaume B card |
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Affiliation: | (1) United States Department of Agriculture, Eastern Regional Research Center, Agricultural Research Service, 600 East Mermaid Lane, 19118 Philadelphia, PA, USA |
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Abstract: | Summary Chelation solubilization of gellan under mild conditions has been accomplished for the first time by exposure to either 10 mM sodium citrate buffer (pH 6.0) or to 1 mM sodium hexametaphosphate (pH 6.6). The citrate system was preferred for most applications since its is a ubiquitous cellular component, its solutions are autoclavable, and because viable plant tissues, fungi, and bacteria could be recovered from culture. Such recovery is not possible from more commonly used media such as agar. |
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