Photoautotrophic shoot and root development for triploid melon |
| |
Authors: | Adelberg Jeffrey Fujiwara Kazuhiro Kirdmanee Chalermpol Kozai Toyoki |
| |
Institution: | (1) Department of Horticulture, Clemson University, Clemson, SC 29634, USA;(2) Laboratory of Environmental Control Engineering, Faculty of Horticulture, Chiba University, Matsudo, Chiba 271, Japan;(3) Graduate School of Agr. and Life Sciences, The University of Tokyo, Tokyo, Japan;(4) Present address: National Science and Technology and Development Agency, National Center for Genetic Engineering and Biotechnology, Rama IV Road, Rashatavee, Bangkok, Thailand |
| |
Abstract: | The aim of this investigation was to establish environmental factors which promote growth and photosynthesis of melon (Cucumis
melo L.) shoot buds, in vitro, and determine if photoautotrophic shoots had superior root forming ability in photoautotrophic
environments. Buds from the triploid melon clone ‘(L-14×B)×L-14’ were observed for 21 days after transfer from a multiplication
MS medium with 3% sucrose and 10 μM benzyladenine (BA) to a shoot development medium with 1 μM BA at three levels of sucrose
in the medium (0, 1 and 3%), and light (50, 100 and 150 PPF) and CO2 (500, 1000 and 1500 ppm) in the culture chamber. More shoot buds were observed with 3% sucrose in the medium. Increased light
and CO2 had a positive interaction with shoot proliferation. Fresh and dry weights were greatest at 3% sucrose, 150 PPF light and
1500 ppm CO2. Shoot buds grew more slowly in sugar-free medium, but fresh and dry weight still doubled over 21 days of culture. Net photosynthetic
rates (NPR) of buds were negative after four days in treatment conditions, but became positive after transfer to fresh, sugar-free
medium. Two triploid genotypes of melon were (1) grown in vitro with sugar (photomixotrophic) and without sugar (photoautotrophic),
(2) rooted in sugar-free media, both in a laboratory controlled environment chamber (in vitro) and a greenhouse acclimatization
unit (ex vitro), and (3) compared for subsequent nursery growth in the greenhouse unit. The genotype ‘(L-14×B)×L-14’ produced
more shoots than ‘(L-14×B)×Mainstream’ in both photomixotrophic or photoautotrophic conditions. ‘(L-14×B)×L-14’ rooted as
well from either photoautotrophic and photomixotrophic shoots but ‘(L-14×B)×Mainstream’ rooted less frequently from photoautotrophic
shoots. Seventy-six percent of the shoots in the laboratory controlled environment chamber were able to root photoautotrophically,
whereas 47% of the shoots in the greenhouse acclimatization unit were rooted. Between 77% and 88% of plantlets from all treatment
combinations survived transfer to the nursery. After growth in the nursery, the sizes of plants (fresh weight, dry weight,
leaf area) were the same for either genotype, from either photoautotrophic or photomixotrophic shoots. Nursery plants that
had been rooted in the laboratory controlled environment chamber were larger than those rooted in the acclimatization greenhouse
chamber.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | Cucumis melo L environmental control microcutting morphogenesis shoot development transplant |
本文献已被 SpringerLink 等数据库收录! |
|