Electron microscopic studies of carboxysomes of Thiobacillus neapolitanus |
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Authors: | Yolande A Holthuijzen Jan F L van Breemen Wil N Konings Ernst F J van Bruggen |
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Institution: | (1) Department of Microbiology, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands;(2) Biochemical Laboratory, University of Groningen, Nijenborgh 16, NL-9747 AG Groningen, The Netherlands |
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Abstract: | The outer part of the carboxysomes of Thiobacillus neapolitanus was examined by electron microscopy using negatively stained, cryo-treated, frozen hydrated and freeze dried specimens. From stereo-micrographs of freeze dried and fixated carboxysomes the three dimensional structure of the carboxysomes was elucidated. The carboxysomes always appear as hexagonal bodies, which possess twelve pentameric planes. This indicates that carboxysomes have the form of a pentagonal dodecahedron. Inside the carboxysomes the ribulose-1,5-bisphosphate carboxylase molecules are arranged in rows and concentric rings. Negatively stained and cryo-treated carboxysomes do not differ significantly in size. The mean size of these carboxysomes is 117.3±6.9 nm (n=782) |
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Keywords: | Carboxysomes Ribulose-1 5-bisphosphate carboxylase Pentagonal dodecahedron |
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