The effect of synthetic and naturally occurring trehalose fatty acid esters in tumor regression

Summary Dimycolates of trehalose, purified cord factor (P3), or monomycolates of trehalose (P4) isolated from various species of mycobacteria, when emulsified with oil droplets and endotoxins from 0 antigen-deficient Re mutants of Salmonella typhimurium or S. minnesota, were found to be effective in regressing transplantable line-10 tumors in strain-2 guinea pigs. Each of these naturally occurring mono- and diesters contains mixtures of several different mycolic acids. To help determine the structural requirements for tumor regression, 6-monoesters and 6,6-diesters of ,-D-trehalose were prepared synthetically and tested. Esters formed with single types of mycolic acids isolated from Mycobacterium tuberculosis strain Brevannes, when combined with endotoxin and oil droplets, had tumor regressive potency equal to that of the more complex natural products. The synthetic 6,6 trehalose diester of 2-eicosyl-3-hydroxy-tetracosanoic (behenylbehenic) acid, whose structure resembles that of mycolic acid but is of lower molecular weight, provided a cure rate of 60%. However, a similar ester prepared with straight chain docosanoic (behenic) acid was essentially inactive.