A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH |
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| Authors: | Price Joseph A |
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| Affiliation: | Department of Pathology, Oklahoma State University Center for Health Sciences, 1111 W. 17th. St. Tulsa, OK 74107-1898, USA. joseph.price@okstate.edu |
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| Abstract: | Phospholipase A(2) is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements. |
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| Keywords: | Lipase PLA2 Phospholipase Bromothymol blue High-throughput Microplate |
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