Promising Biological Indicator of Heavy Metal Pollution: Bioluminescent Bacterial Strains Isolated and Characterized from Marine Niches of Goa,India |
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Authors: | Neha A Thakre Arti S Shanware |
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Institution: | Rajiv Gandhi Biotechnology Centre, Rashtrasant Tukadoji Maharaj Nagpur University, L.I.T. Campus, Amravati Road, Nagpur, 440033 Maharashtra India |
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Abstract: | In present study, several marine water samples collected from the North Goa Beaches, India for isolation of luminescent bacterial species. Isolates obtained labelled as DP1-5 and AB1-6. Molecular characterization including identification of a microbial culture using 16S rRNA gene based molecular technique and phylogenetic analysis confirmed that DP3 & AB1 isolates were Vibrio harveyi. All of the isolates demonstrated multiple metal resistances in terms of growth, with altered luminescence with variable metal concentration. Present investigations were an attempt towards exploring and reporting an updated diversity of bioluminescent bacterial species from various sites around the Goa, India which would be explored in future for constructing luminescence based biosensor for efficiently monitoring the level of hazardous metals in the environment.Electronic supplementary materialThe online version of this article (doi:10.1007/s12088-015-0531-y) contains supplementary material, which is available to authorized users. |
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Keywords: | Heavy metals pollution Biosensor Vibrio harveyi Bioluminescent bacteria 16S rRNA gene analysis Bioassay Monitoring |
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