Stimulation of Proliferation and Migration of Mouse Macrophages by Type B CpG-ODNs Is F-Spondin and IL-1Ra Dependent |
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| Authors: | Tai-An Chen Chiao-Chun Liao Yung-Chih Cheng Yen-Po Chen Yi-Fan Hsu Chi-Ming Liang Shu-Mei Liang |
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| Institution: | 1. Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.; 2. Genomics Research Center, Academia Sinica, Taipei, Taiwan.; Rutgers—New Jersey Medical School, UNITED STATES, |
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| Abstract: | Macrophage proliferation and migration are important for many facets of immune response. Here we showed that stimulation of macrophages with type B CpG oligodeoxynucleotides (CpG-B ODNs) such as CpG-ODN 1668 increased the production of anti-inflammatory cytokine interleukin 1 receptor antagonist (IL-1Ra) in a TLR9- and MyD88-dependent manner. The CpG-B ODNs-induced IL-1Ra increased macrophage migration and promoted macrophage proliferation by down-regulating the expression of a cell cycle negative regulator, p27 to increase cell population in the S phase. The induction of IL-1Ra by CpG-B ODNs was F-spondin dependent. Knockdown of F-spondin and IL-1Ra decreased CpG-B ODNs-induced macrophage migration whereas overexpression of IL-1Ra increased migration of those cells. These findings demonstrated novel roles for F-spondin and IL-1Ra in CpG-B ODNs-mediated cell proliferation and migration of macrophages. |
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