Molecular cloning of tobacco chloroplast ribosomal RNA genes |
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Authors: | Masahiro Sugiura and Jun Kusuda |
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Institution: | (1) National Institute of Genetics, 411 Mishima, Japan |
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Abstract: | Summary Tobacco chloroplast ribosomal RNAs were shown to be hybridized with two EcoRI fragments of tobacco chloroplast DNA. These DNA fragments having molecular weights of 1.9x106 and 2.8x106 daltons were cloned using the bacterial plasmid pMB9 as a vector and E. coli HB101 as host bacteria. The recombinant plasmids containing either or both of these fragments were constructed and characterized.Abbreviations rRNA
ribosomal RNA
- EDTA
ethylenediamine tetraacetic acid
- SSC
0.15 M NaCl-0.015 M sodium citrate
- EcoRI and HindIII
restriction endonucleases isolated from E. coli RY13 and Haemophilus influenzae Rd, respectively |
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Keywords: | |
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