Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants. |
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| Authors: | P Schmieder J H Ippel H van den Elst G A van der Marel J H van Boom C Altona H Kessler |
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| Affiliation: | Organisch-Chemisches Institut, TU München, Garching, Germany. |
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| Abstract: | Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure. |
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