忍冬木层孔菌液体培养过程中多酚含量及抗氧化活性研究

忍冬木层孔菌是木层孔菌属的一种药用真菌，目前对它的研究少有报道。以液体培养为基础，研究忍冬木层孔菌培养过程中生物量、胞内外多酚含量的变化，以及胞内外提取物抗氧化活性的变化。结果显示，菌丝体生物量在培养168–216h时达到最大值（8.33g/L）；胞内多酚和胞外多酚含量分别在216h和168h时达到最大值（15.60mg GAE/g和106.76mg GAE/L）；液体培养胞内外提取物的抗氧化活性与多酚含量正相关，多酚含量高时提取物对DPPH1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl]自由基的抑制率也高。

Polyphenol content and antioxidant activities of Phellinus lonicericola in liquid culture

Phellinus lonicericola is a medicinal fungus belonging to the Hymenochaetaceae. Few reports on the fungus were published previously. The changes of biomass and polyphenolic content in mycelia (intracellular polyphenol IP) and culture broth (extracellular polyphenol EP), and the varieties of antioxidant activities of mycelial extracts and culture broth were studied during the liquid culture of P. lonicericola. The results showed that the highest levels for mycelial biomass of P. lonicericola reached 8.33g/L at 168 and 216h, for IP 15.60mg GAE/g at 216h and EP 106.76mg GAE/L at 168h. The extracts of the mycelia showed antioxidant activity against DPPH radical with capacity positively related to the polyphenolic content.