IL-8通过激活PKC/ERK信号通路促进肾癌细胞上皮细胞-间质细胞转化

上皮细胞-间质细胞转化(EMT)在肿瘤转移方面起着非常重要的作用．肾癌发生EMT的具体分子机制尚不清楚．IL-8是一个重要的炎症趋化因子，研究表明肾癌细胞可以分泌IL-8，但IL-8是否参与肾癌细胞EMT的调节目前尚无报道．我们研究发现，IL-8可以促进肾癌细胞形态发生间质化改变，IL-8刺激后E-钙黏蛋白表达水平下降， N-钙黏蛋白表达上调．另外，IL-8可以促进肾癌细胞侵袭，但对肾癌细胞增殖的影响并不明显．进一步研究显示，IL-8通过激活蛋白激酶C(PKC)引起细胞外调节性激酶(ERK)磷酸化．因此，我们认为IL-8可能通过PKC/ERK信号通路促进肾癌细胞发生EMT，这可能是肾癌转移的重要机制之一．

IL-8 Regulates The Epithelial-mesenchymal Transition (EMT) of Renal Cancer Through PKC/ERK Signaling Pathway

The epithelial-mesenchymal transition(EMT) is an essential component in tumor metastasis.However,the molecular mechanism of EMT in renal cancer is unclear.IL-8 is an important chemokine in tumor microenvironment.Studies have shown that renal cancer cells can secrete IL-8,whether it could induce EMT in renal cancer cells is unknown.Here we found that IL-8 could induce EMT in renal cancer cells.Upon the stimulation of IL-8,the expression of E-cadherin was up-regulated,while the expression of N-cadherin was down-regulated;IL-8 promoted the invasion of renal cancer cells,but there was no obvious impact on cell proliferation.In addition,IL-8 could activate ERK through PKC.Therefore,we believe that IL-8 may promote renal cancer EMT through PKC / ERK signaling pathway,which may be one of the important mechanisms of renal cancer metastasis.