| 桦褐孔菌菌丝体及甾类化合物的发酵条件优化 |
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| 引用本文： | 黄伟,陆震鸣,耿燕,许泓瑜,史劲松,许正宏. 桦褐孔菌菌丝体及甾类化合物的发酵条件优化[J]. 菌物学报, 2012, 31(6): 909-916 |
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| 作者姓名： | 黄伟 陆震鸣 耿燕 许泓瑜 史劲松 许正宏 |
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| 作者单位： | 1. 江南大学医药学院制药工程研究室 江苏无锡214122
2. 江南大学医药学院生物活性制品加工工程研究室 江苏无锡214122 |
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| 基金项目： | 国家“863”重点项目（No. 2011AA02A211）；江苏省自然科学基金（No. BK2010142）；江苏省产学研联合创新资金项目（No. BY2012052）；中央高校基础研究项目（Nos. JUSRP211A42, JUSRP211A43） |
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| 摘 要： | 对桦褐孔菌深层发酵培养基进行了筛选，以菌丝体及甾类化合物产量为目标对发酵条件进行了优化，确定最佳发酵条件为：30g/L葡萄糖，2.5g/L黄豆粉，2.5g/L蛋白胨，3g/L KH2PO4，0.8g/L MgSO4，0.8g/L CaSO4，初始pH4.0，接种量15%，装液量100mL/500mL，转速150r/min，28℃恒温培养。此条件下培养11d，菌丝体干重达12.52g/L，甾体类化合物的产量达112.44mg/L。
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| 关 键 词： | 桦褐孔菌 最佳发酵培养基 初始pH 接种量 |
Optimization of submerged fermentation condition for production of mycelial biomass and steroids by Inonotus obliquus |
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| HUANG Wei,LU Zhen-Ming,GENG Yan,XU Hong-Yu,SHI Jin-Song and XU Zheng-Hong. Optimization of submerged fermentation condition for production of mycelial biomass and steroids by Inonotus obliquus[J]. Mycosystema, 2012, 31(6): 909-916 |
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| Authors: | HUANG Wei LU Zhen-Ming GENG Yan XU Hong-Yu SHI Jin-Song XU Zheng-Hong |
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| Affiliation: | Laboratry of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, China;Laboratry of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, China;Laboratry of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, China;Laboratry of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, China;Laboratry of Bioactive Products Processing Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, China;Laboratry of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu 214122, China |
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| Abstract: | To produce mycelial biomass and steroids, the optimization of submerged fermentation condition for Inonotus obliquus was carried out. The optimum fermentation medium was as follows: 30g/L glucose, 2.5g/L Soybean powder, 2.5g/L peptone, 3g/L KH2PO4, 0.8g/L MgSO4, 0.8g/L CaSO4. The initial pH was 4.0, and the inoculation volume was 15%. The fungus was cultured at 28℃ in 500mL shake flasks containing 100mL of medium at 150r/min. Under this condition, mycelial biomass of 12.52g/L was obtained while production of steroids about 112.44mg/L. |
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| Keywords: | Inonotus obliquus optimum fermentation medium initial pH inoculation volume |
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