| 一株产生L-乳酸的米根霉 |
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| 引用本文： | 周倩,赵龙,蒋雪薇,罗晓明,边佳为.一株产生L-乳酸的米根霉[J].菌物学报,2012,31(6):956-962. |
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| 作者姓名： | 周倩 赵龙 蒋雪薇 罗晓明 边佳为 |
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| 作者单位： | 1. 湖南农业大学生物安全科学技术学院 湖南长沙410128
2. 长沙理工大学化学与生物工程学院 湖南长沙410004 |
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| 基金项目： | 湖南省教育厅资助项目（No. 06C090）；湖南师范大学微生物分子生物学湖南省重点实验室开放基金（No. MM0802） |
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| 摘 要： | 为获得理想的L-乳酸产生菌，选择适合根霉属微生物生长的土样，利用溴甲酚绿平板结合摇瓶复筛的方法得到了一株有一定L-乳酸积累能力的米根霉Rhizopus oryzae CS323。摇瓶发酵试验显示，在未优化发酵条件的情况下发酵48h，米根霉CS323L-乳酸积累量达到50.1g/L，是一株有良好改造潜力的L-乳酸产生菌，适合作为进一步诱变育种的出发菌株。
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| 关 键 词： | 米根霉 溴甲酚绿平板 摇瓶发酵试验 |
A Rhizopus oryzae strain producing L-lactic acid |
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| Authors: | ZHOU Qian ZHAO Long JIANG Xue-Wei LUO Xiao-Ming and BIAN Jia-Wei |
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| Institution: | College of Bio-Safety Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China;College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410004, China;College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410004, China;College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410004, China;College of Chemical and Biological Engineering, Changsha University of Science and Technology, Changsha, Hunan 410004, China |
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| Abstract: | In order to obtain an ideal strain producing L-lactic acid, Rhizopus oryzae strain CS323 was obtained by bromocresol green agar plate and shaking flask screening from soil samples. Shaking flask test showed that, without optimization of fermentation conditions, L-lactic acid accumulation of the strain CS323 was 50.1g/L after 48h fermentation. It was a potential L-lactic acid producer suitable for further mutation. |
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| Keywords: | Rhizopus oryzae bromocresol green agar shaking flask test |
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