红富士苹果园酵母多样性研究

从北京顺义和山东泰安红富士苹果园采集果实、叶片、树皮和土壤等不同基物，分离酵母菌，利用26S rDNA的D1/D2区域序列分析并结合形态学特征和SSCP分析对这些菌株进行了分类学研究，探讨了苹果园酵母的物种多样性及其分布。北京苹果园共分离酵母菌129株，鉴定为13属21种，优势属为Pichia（4个种），Cryptococcus（3个种），Pseudozyma（3个种），子囊菌占较大优势，分布于8属12种，占总种数的57.1%。山东苹果园共分离酵母291株，鉴定为13属26种，优势属为假丝酵母Candida（6个种），毕赤酵母Pichia（4个种）和隐球酵母Cryptococcus（3个种），并且子囊菌占较大优势，分布于7属17种，占总种数的65.4%。

Species diversity of yeasts in Fuji apple orchards

Yeast strains were isolated from fruit, leaf and soil samples collected from Fuji apple orchards in Shunyi, Beijing and Tai’an, Shandong Province. Yeast strains were identified based on the combination of morphology and 26S rDNA Dl/D2 domain sequence analysis. A total of 129 yeast strains belonging to 21 species in 13 genera were isolated from samples in Beijing. The dominant genera were Pichia (4 species), Cryptococcus (4 species) and Pseudozyma (3 species). Among the yeast species identified, 57.1% were ascomycetous yeasts belonging to 12 species in 8 genera. A total of 291 yeast strains belonging to 26 species in 13 genera were isolated from samples in Shandong Province. The dominant genera were Candida (6 species), Pichia (4 species) and Cryptococcus (3 species). Ascomycetous yeast species were also dominant, accounting for 65.4% (17 species belonging to 7 genera) of the species identified from the Shangdong strains.