低温高效苯酚降解菌的分离与降解特性

从哈尔滨太平污水厂活性污泥中筛选到7株高效苯酚降解菌,可利用苯酚作为唯一碳源和能源。通过对这7株菌在不同温度、pH值、以及不同苯酚浓度下生长和苯酚降解情况的考察,确定了这7株菌的最适生长温度为10°C,最适pH值为7.5,最大可降解苯酚浓度为3000mg/L。通过对这7株苯酚降解菌降解性能的研究表明:其具有较强的苯酚降解能力,在10°C、pH值为7.5、装液量为50mL、接种量15%、摇床振荡速度160r/min的条件下,反应48h后可使500mg/L的苯酚降解率达90%以上。葡萄糖对菌体的生长及苯酚降解能力均有一定的影响,当葡萄糖浓度是500mg/L时,该菌对苯酚的降解率仍在80%以上。该研究对处理含有其它碳源的含酚废水具有一定的意义。通过DGGE图谱条带的分析表明,其亮度可以说明这些菌在各个系统中均表现为优势菌,且在污水环境中表现出较强的活性,其优势地位能够稳定地存在。其中2、4、24、28条带丰富,表现出它们在污水环境系统中的多样性。

Efficient Low-temperature Separation of Phenol-degrading Bacteria and Degradation Characteristics

Screening seven strains of efficient phenol degradation bacterium in activated sludge from Harbin Taiping sewage treatment plant. These strains can use phenol as the only available carbon sources and energy. Based on inspects the growth and degradation of phenol in different temperature, pH value, and different phenol concentration. We can determine that the optimal temperature of seven bacterias is 10oC, the optimal pH value is 7.5, the lagest concentration of degradation of phenol is 3000 mg/L. From the study of degradation performance by these seven bacteriums shows that it has strong ability of phenol degradation. In the condition of 10°C, pH 7.5, fluid for 50 mL, vaccination is 15%, vibration velocity wave 160 r/min, degradation rate of 500 mg/L phenol can reach more than 90% after 48 h in the training. Glucose to the growth of bacteria and phenol degradation ability all have certain effects, when glucose concentration is 500 mg/L, the degradation rate of phenol is still more than 80%. The study on treatment contain other carbon sources of hydroxyl benzene wastewater has certain significance. Through the analysis DGGE strip map shows that the brightness can explain these bacteria are advantage bacterium in each system, and strong activityin sewage, its dominant position can be exist steadily. Rich strips are 2, 4, 24, 28, and it is showing its diversity in sewage system.