Prostatic growth factor: purification and structural relationship to basic fibroblast growth factor |
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Authors: | M T Story J Sasse S C Jacobs R K Lawson |
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Institution: | Department of Urology, Medical College of Wisconsin, Milwaukee 53226. |
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Abstract: | Prostatic growth factor (PrGF) was purified from alkaline homogenates of human benign prostatic hyperplastic tissue by a combination of ammonium sulfate precipitation, heparin affinity chromatography, and cation-exchange chromatography. The 17,600-dalton, basic (pI 10.2) PrGF is related to basic fibroblast growth factor (bFGF) since antisera raised against synthetic peptides with sequence homologies corresponding to an internal peptide and amino- and carboxyl-terminal peptides of bFGF react with the growth factor. The growth factor appears larger than bFGF, suggesting that additional amino-terminal sequences may be present as a result of alkaline extraction in the presence of protease inhibitors. |
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