Regeneration of whole plants from callus culture of diverse genetic lines of Pisum sativum L. |
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Authors: | Russell L Malmberg |
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Institution: | (1) Department of Crop and Soil Sciences, Michigan State University, 48824 East Lansing, MI, USA |
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Abstract: | Sixteen genetic lines of peas were screened for their ability to regenerate whole plants from callus cultures. Epicotyl sections from germinating seeds were placed on callus-inducing medium; the resulting callus was subcultured monthly and was tested every other month for its regeneration ability. Six lines were found that would regenerate after 2 months' growth as callus. Four of these continued to regenerate after 4 months and, of these, two after 6 months. The cultivars Frosty and Alaska were among the lines that would not regenerate at all.Michigan Agricultural Experiment Station Journal Article No. 8932 |
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Keywords: | Callus culture Genetic lines (Pisum) Pisum Regeneration (callus cultures) Shoot regeneration |
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