Behavior of various ribo- and deoxyribonucleosides, nucleoside monophosphate kinases, and nucleoside diphosphokinase on Blue Sepharose affinity columns. |
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| Authors: | Y C Cheng B Domin |
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| Affiliation: | 1. Department of Experimental Therapeutics, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14263 USA;2. Grace Cancer Drug Center, Roswell Park Memorial Institute, New York State Department of Health, Buffalo, New York 14263 USA |
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| Abstract: | A rapid and effective separation and purification of cytidylate-deoxycytidylate-uridylate kinase, adenylate kinase, and nucleoside diphosphokinase has been achieved with Blue Sepharose CL6B chromatography. When crude extracts of human crythrocytes or acute myelocytic leukemia cells were applied to the column, adenylate kinase, cytidylate-deoxycytidylate-uridylate kinase, and nucleoside diphosphokinase were retarded while guanylate kinase, cytidine kinase, uridine kinase, and deoxycytidine kinase were unabsorbed. The buffers required to elute the retarded kinases depended on the amount of sample applied to the column. |
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| Keywords: | Author to whom reprint requests should be addressed. Dr. Cheng is a Leukemia Society Scholar. |
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