Studies on Morphological Transformation of BALB/3T3-Derived Clones by Murine Leukemia Virus |
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| Authors: | Helene S Smith Adeline J Hackett E Louise Springer and John Riggs |
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| Institution: | *Cell Culture Laboratory, School of Public Health, University of California-Berkeley, Berkeley, California 94720;.California State Department of Public Health Viral and Rickettsial Disease Laboratory, Berkeley, California 94720 |
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| Abstract: | We have described a cell line, UC1-B, derived spontaneously from BALB/3T3 mouse embryo cells, which, unlike the standard BALB/3T3, are morphologically transformed and produce bizarre viral forms in response to murine leukemia virus. Although UC1-B and BALB/3T3 are morphologically similar, and both form contact-inhibited monolayers at confluence, the UC1-B cells are partially transformed because: they grow to a slightly higher saturation density than 3T3 cells, they grow in medium lacking serum growth factors, and they produce tumors in mice. Another clone, 12A-3, derived from BALB/3T3, also transforms and produces bizarre viral forms after infection with murine leukemia virus. Unlike UC1-B cells, the 12A3-8 cells are identical in growth properties to BALB/3T3; therefore, a partially altered morphology is not required for the induction of transformation by murine leukemia virus. |
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