The immune response against Francisella tularensis live vaccine strain in Lpsn and Lpsd mice |
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Authors: | Ales Macela Jiri Stulik Lenka Hernychova Michal Kroca Zuzana Krocova Hana Kovarova |
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Affiliation: | Institute for Immunology, Purkyne Military Medical Academy, 502 60 Hradec Kralove, Czech Republic |
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Abstract: | Abstract The impact of Lps gene on the course of immune response against subcutaneous infection of mice with Francisella tularensis live vaccine strain was studied. Production and specificity of antibodies, cytotoxic responses of macrophages and NK-cells, spontaneous production ex vivo of cytokines IL-1α, IL-2, IL-4, IL-6, IL-10, IFN-γ, and TNF-α in spleen cell cultures in C3H/HeJ ( Lps d) mice in comparison with C3H/HeN ( Lps r) mice were tested. The value of LD50 was significantly different in the two strains of mice (8.0 × 103 cfu for C3H/HeJ versus 4.61 × 105 cfu for C3H/HeN mice after subcutaneous inoculation). The production of NO2 is also impaired in C3H/HeJ mice in the early intervals after infection. Thus, the defective Lps gene of C3H/HeJ mice influences both the level of innate resistance of mice to F. tularensis live vaccine strain infection and the process of induction and regulation of immune response against this intracellular bacterial pathogen. |
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Keywords: | Francisella tularensis LPS gene Antibody Cytokine Cytotoxic activity N-Oxide |
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