An improved electron microscopic technique for the immunolabeling of Cryptosporidium parvum oocysts |
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| Authors: | Jenkins Mark C Murphy Charles Trout James Fayer Ronald |
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| Affiliation: | Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, USA. mjenkins@anri.barc.usda.gov |
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| Abstract: | A technique was developed for immunolabeling Cryptosporidium parvum oocysts for subsequent observation by transmission electron microscopy. This method was developed to maintain architectural integrity of the oocyst wall and improve fixation of internal contents. The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa. |
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