The fate of embryonal-carcinoma cells in mouse blastocysts |
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Authors: | G. Barry Pierce Juan Arechaga Alan Jones rea Lewellyn Robert S. Wells |
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Affiliation: | Department of Pathology, University of Colorado School of Medicine. 4200 East Ninth Avenue. Denver, Colorado 80262, USA |
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Abstract: | Double-labeled embryonal-carcinoma (ECa) cells were injected into blastocysts or incorporated into blastocysts by aggregation, and their fate after various periods of time in culture was investigated. ECa-247 cells labeled with fluorescent microscopheres were easily identified in whole blastocysts. These blastocysts were embedded in plastic, serially sectioned, and prepared for autoradiography. The 3H-thymidine label on the embryonal-carcinoma cells allowed precise localization of the cancer-derived cells. ECa-247 cells preferentially localized in the mural trophectoderm, with a few being seen in primitive endoderm and, even more rarely, in the inner cell mass. Selected autoradiograms were re-embedded and thin sectioned for transmission electron microscopy. The cancer-derived cells were found to have differentiated in accordance with their localization. |
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