Genetic divergence and phylogenetic analysis of genus <Emphasis Type="Italic">Jatropha</Emphasis> based on nuclear ribosomal DNA ITS sequence |
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Authors: | D V N Sudheer Pamidimarri Balaji Chattopadhyay Muppala P Reddy |
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Institution: | (1) Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (CSIR), G. B. Marg, Bhavnagar, Gujarat, 364002, India;(2) Department of Animal Behaviour and Physiology, School of Biological Science, Madurai Kamaraj University, Madurai, Tamilnadu, 625021, India |
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Abstract: | The genus Jatropha belongs to the family Euphorbiaceae having significant economic importance. The present investigation was undertaken with
an aim to understand phylogenetic relationships among seven species (J. curcas, J. glandulifera, J. gossypifolia, J. integerrima, J. multifida, J. podagrica, and J. tanjorensis.) which are widely distributed in India, using nuclear ribosomal DNA ITS sequence (nrDNA ITS) and to compare the results
with multilocus marker analysis systems reported earlier for the same genus. The size variation obtained among sequenced nrDNA
ITS regions was narrow and ranged from 647 to 654 bp. The overall mean genetic distance (GD) of genus Jatropha was found to be 0.385. Highest interspecific GD (0.419) was found between J. glandulifera and J. multifida. The least interspecific GD (0.085) was found between J. gossypifolia and J. tanjorensis. The highest intraspecific GD was observed in J. podagrica (0.011) and least in J. gossypifolia (0.002). The phylogram obtained using nrDNA ITS sequence showed congruence with the phylograms obtained using multilocus
markers system reported earlier with minor variations. The present study also strongly supports high phylogentic closeness
of J. curcas and J. integerrima. The only exception found was J. podagrica which clustered with J. multifida in earlier based on multilocus marker analysis, was clustered with J. curcas in the present analysis. The sequence data generated in the present investigation will help for further studies in intraspecies
population, and their phylogentic analysis, biogeographical, molecular evolution studies and also pave way for future phylogetic
and/or evolution studies among the other groups belongs to the family Euphorbiaceae. |
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Keywords: | Genetic divergence Phylogeny Jatropha and nrDNA ITS region |
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