| 阿魏菇多糖高产菌的离子束和激光复合诱变育种 |
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| 引用本文： | 陈恒雷,武宝山,石伟娜,曾宪贤.阿魏菇多糖高产菌的离子束和激光复合诱变育种[J].生物技术,2010,20(1):30-33. |
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| 作者姓名： | 陈恒雷 武宝山 石伟娜 曾宪贤 |
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| 作者单位： | 1. 新疆大学物理学院离子束生物工程中心,新疆,乌鲁木齐,830008南;京工业大学制药与生命科学学院,江苏,南京,210009
2. 新疆大学物理学院离子束生物工程中心,新疆,乌鲁木齐,830008 |
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| 基金项目： | 新疆大学青年教师科研启动基金项目，国家发改委高技术产业化项目 |
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| 摘 要： | 目的:诱变筛选阿魏菇多糖高产菌,探索食用菌的离子束和激光复合诱变育种方法。方法:尝试用阿魏菇菌丝单细胞为靶材,以离子束注入和激光辐照为复合诱变手段,采用营养缺陷型筛选办法定性初筛,摇瓶发酵定量复筛。结果:通过离子束注入诱变,获得了2株菌丝体多糖产量分别达到551.80mg/L和659.46mg/L、较1号出发菌株提高了46.5%和75.2%的多糖高产菌PFPH-1和PFPH-2;在此基础上,以激光辐照为复合诱变手段,获得了1株菌丝体多糖产量达到762.50 mg/L、较出发菌株PFPH-2提高了15.63%的多糖高产菌PFPH-3。结论:离子束和激光复合诱变育种方法在改良阿魏菇多糖高产性状方面诱变功效显著。
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| 关 键 词： | 阿魏菇多糖 高产菌 离子柬 激光 复合诱变育种 |
Ion Beam and Laser Composite Mutagenesis Breeding of Pleurotus Polysaccharide High-yield Strains |
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| CHEN Heng-lei,WU Bao-shan,SHI Wei-na,ZENG Xian-xian.Ion Beam and Laser Composite Mutagenesis Breeding of Pleurotus Polysaccharide High-yield Strains[J].Biotechnology,2010,20(1):30-33. |
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| Authors: | CHEN Heng-lei WU Bao-shan SHI Wei-na ZENG Xian-xian |
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| Institution: | CHEN Heng-lei1,2,WU Bao-shan1,SHI Wei-na1,ZENG Xian-xian1(1.Ion Beam Bio-engineering Center,School of Physics Science , Technology,Xinjiang University,Urumqi 830008,2.College of Life Science , Pharmacy Engineering,Nanjing University of Technology,Nanjing 210009,China) |
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| Abstract: | Objective:To obtain Pleurotus polysaccharide high-yield strains and explore an ion beam and laser composite mutagenesis breeding method for edible fungus.Method:Mycelium mono-cells of Asafoetida mushroom were treated tentatively by ion beam and laser composite mutagenesis through fermentation quantitative screening after auxotrophy qualitative primary screening.Result:Two polysaccharide high-yield strains,PFPH-1 and PFPH-2,were selected by ion beam implantation.The polysaccharide yield of these mutants was ... |
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| Keywords: | Pleurotus polysaccharide high -yield strains ion beam laser composite mutagenesis breeding |
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