Differentiation of metaxylem cell line in the root ofAllium cepa |
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| Authors: | R Cremonini M lo Iero M Durante |
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| Institution: | (1) Institute of Botany, The University, Pisa;(2) Institute of Genetics, The University, Via Matteotti 1 A, 1-56100 Pisa, Italy |
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| Abstract: | Summary The first step of differentiation in the root segments ofAllium cepa containing metaxylem cells in different stages of differentiation were studied by DNA reassociation curves and compared to meristem cell extracted DNA. Upon sonication of DNA samples to about 400 base pairs, the reassociation profiles of the heat denatured DNA, were spectrophotometrically followed at two different concentrations. The kinetic complexities,i.e., the number of base pairs per haploid genome of a given sequence and its redundancy were calculated. Differences were found at the level of highly and medium repetitive sequences, thus demonstrating that some DNA reassociation classes may undergo amplification during root development. |
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| Keywords: | Allium cepa Metaxylem Differentiation DNA repetitive sequences |
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