A simple and reliable EDDA method for mycobactin production in mycobacteria: Optimal conditions and use in mycobacterial speciation |
| |
| Authors: | Dr Suzana Bosne Fernand Papa Sabine Clavel-Sérès Nalin Rastogi |
| |
| Institution: | (1) Tuberculosis and Mycobacteriology Unit, Institut Pasteur, Paris, France |
| |
| Abstract: | Ethylenediamino-di-(0-hydroxyphenylacetic acid) (EDDA) was found to be a suitable iron chelator for investigating mycobactin production in mycobacteria. The optimal conditions of EDDA concentration and time of exposure were established and were used to develop a twostep method for mycobactin production. Applied to representative strains of selected species, the method was found to yield reliable results useful for identification and speciation of mycobacteria. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
