CEREBROSPINAL FLUID HOMOVANILLIC ACID AND ISO-HOMOVANILLIC ACID: A GAS–LIQUID CHROMATOGRAPHIC METHOD |
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| Authors: | S W Dziedzic S E Gitlow |
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| Institution: | Department of Medicine of the Mount Sinai School of Medicine, New York, NY 10029, U.S.A. |
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| Abstract: | Abstract— The hexafluoroisopropyl esters of trifluoroacetylated homovanillic acid (HVA) and 3-hydroxy-4-methoxyphenylacetic acid (iso-HVA) were the derivatives chosen for assay of these phenolic acids in lumbar cerebrospinal fluid (CSF) specimens from 65 control subjects. The mean value obtained in these specimens was 40.0 ± 23.5 ng/ml. Recovery of added HVA and iso-HVA was 94 ± 2 per cent and reproducibility was ±5 per cent. CSF from Parkinson's disease patients undergoing treatment with l -DOPA showed significant increases in HVA. Iso-HVA was detected in those CSF samples obtained during l -DOPA therapy. |
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