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Nopaline synthase gene is expressed in Escherichia coli and in cucumber cells under a hybrid promoter
Authors:Yedidya Gafni
Institution:(1) Department of Plant Genetics and Breeding, Agricultural Research Organization, The Volcani Center, P.O. Box 6, 50250 Bet Dagan, Israel
Abstract:Fusion of the nopaline synthase gene (nos) to the Escherichia coli trp promoter gave rise to a hybrid promoter (tros). Under control of this hybrid element, synthesis of nopaline was observed in E. coli as well as in cucumber cells transformed with the described vector.This paper is contribution no. 2698-E, 1989 series from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Keywords:Agrobacterium tumefaciens  trp promoter  Cucumis sativus  plasmids  binary vectors  nopaline
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