Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean |
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| Authors: | Takashima Yoshinori Senoura Takeshi Yoshizaki Takayuki Hamada Shigeki Ito Hiroyuki Matsui Hirokazu |
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| Affiliation: | Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. |
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| Abstract: | Plant isoamylase-type starch-debranching enzymes (ISAs) hydrolyze alpha-1,6-linkages in alpha-1,4/alpha-1,6-linked polyglucans. Two ISAs, designated PvISA1/2 and PvISA3, were purified from developing seeds of kidney bean by ammonium sulfate fractionation and several column chromatographic procedures. The enzymes displayed different substrate specificities for polyglucans: PvISA1/2 showed broad chain-length specificities, whereas PvISA3 liberated specific chains with a DP of 2 to 4. |
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