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Quantitative trait loci affecting seed mineral concentrations in Brassica napus grown with contrasting phosphorus supplies
Authors:Guangda Ding  Mei Yang  Yifan Hu  Yuan Liao  Lei Shi  Fangsen Xu  Jinling Meng
Institution:1National Key Laboratory of Crop Genetic Improvement;2Microelement Research Centre, Huazhong Agricultural University, Wuhan 430070, China
Abstract:MethodsA population of 124 recombinant inbred lines derived from a cross between P-inefficient ‘B104-2’ and P-efficient ‘Eyou Changjia’ was used for phenotypic investigation and QTL analysis. Two-year field trials were conducted with two P treatments. Concentrations of mineral elements (P, Ca, Mg, Fe, Zn, Cu and Mn) in seeds were determined and QTLs were identified by composite interval mapping.ConclusionsThe accumulation of mineral elements in seeds is controlled by multiple genes. Common physiological and molecular mechanisms could be involved in the accumulation of several mineral elements, and genes involved in these processes in B. napus are suggested. These results offer insights to the genetic basis of seed mineral accumulation across different P levels in B. napus.
Keywords:Brassica napus  phosphorus deficiency  seed mineral concentration  quantitative trait locus  in silico mapping  recombinant inbred line
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