交联蛋白晶体技术及其应用

作为蛋白工程的一项突破性技术,交联蛋白晶体技术正受到广泛重视。蛋白晶体的交联形式不仅保留了蛋白的生物活性,而且显示了对热、极端pH和温度、有机溶剂和蛋白水解酶的更高稳定性。本文介绍交联蛋白晶体的制备和特性及其在生物医学、有机合成、环境催化和色谱分析中的应用 。

Cross-linked Protein Crytals Technology and Its Applications

As a breakthrough technology in protein engineering,cross|linked protein crystals technology is attracting wide attentions.The cross|linked forms of protein crystals not only retain the bioactivity,but also display enhanced stability against heat,extremes of pH and temperature,organic solvents and proteolysis.In this review,the preparation and properties of cross|linked protein crystals and their applications in biomedicine,organic synthesis,environmental catalysis and chromatographic analysis were introduced.