Differentiation of myoblasts is accelerated in culture in a magnetic field |
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Authors: | Louis Yuge Katsuko Kataoka |
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Institution: | (1) Institute of Health Sciences Faculty of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8551 Hiroshima, Japan;(2) Department of Anatomy, Faculty of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8551 Hiroshima, Japan |
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Abstract: | Summary We developed a new cell stimulation method in which magnetic microparticles (MPs) were introduced into the cytoplasm of cultured
myoblasts and the cells were cultured in a magnetic field. The differentiation of myoblasts was examined from the viewpoint
of their morphology and myogenin production. After exposure to the magnetic field, the cells containing MPs became larger
and were elongated along the axis of the magnetic poles. Myogenin, a muscle-specific regulatory factor involved in controlling
myogenesis, was formed earlier, and myotubes were seen earlier and more frequently in this group of myoblasts than in the
other groups (cells alone without magnetic field, cells containing MPs but without magnetic field, and cells alone with magnetic
field). Moreover, we succeeded in differentiation of early muscle cells with striated myofibrils in culture at 0.05 T. The
precisely quantitative and stable stimulus induced by a magnetic field developed in the present study offers a new approach
to elucidate the entire process of myoblast differentiation into myotubes. |
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Keywords: | cellular models myoblasts magnetic microparticles magnetic field cell differentiation |
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