Transformation of bacteria by electroporation |
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| Institution: | 1. University of Ljubljana, Faculty of Electrical Engineering, Trzaska 25, 1000 Ljubljana, Slovenia;2. University of Zaragoza, Faculty of Veterinary, Zaragoza, Spain;1. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, USA;2. Department of Mechanical Engineering, The Pennsylvania State University, University Park, PA, USA;3. Department of Urology, Stanford University School of Medicine, Stanford, CA, USA.;4. Departmemt of Pathology and Laboratory Medicine, Penn State Milton S. Hershey Medical Center, Hershey, PA, USA;5. Departments of Pediatrics and Public Health Sciences, Penn State University College of Medicine, Hershey, PA, USA;6. GE Global Research, Niskayuna, NY, USA;7. Departments of Mechanical Engineering and Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA;8. Department of Surgery, Penn State Milton S. Hershey Medical Center, Hershey, PA, USA |
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| Abstract: | The introduction of DNA into bacteria by transformation is an essential step in the construction of recombinant strains. Recently, electroporation, or electropermeabilization, in which a brief high voltage electric discharge is used to render cells permeable to DNA, has revolutionized the transformation of bacteria. The technique is fast, simple, reproducible, frequently gives very high transformation frequency and appears to be applicable to a wide range of bacterial types previously thought untransformable. The technique can also offer advantages for transformable bacteria such as Escherichia coli. |
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