Isolation and purification of peptide and alkaloid toxins from nabaena flos-aquae using high-performance thin-layer chromatography |
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| Institution: | 1. NOAA/National Ocean Service/National Centers for Coastal Ocean Science, Charleston, SC, United States;2. University of California Santa Cruz, Santa Cruz, CA, United States;1. Department of Medical Biochemistry, Faculty of Medicine in Hradec Králové, Charles University, Šimkova 870, Hradec Kralove 500 38, Czech Republic;2. ADINACO Research Group, Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University in Prague, Heyrovského 1203, Hradec Králové 500 05, Czech Republic |
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| Abstract: | A procedure has been developed for the sequential extraction and purification of the peptide and alkaloid toxins from a single batch of Anabaena flos-aquae cells. After solvent extraction and adsorption onto C18 Sep-Pak cartridges, the cyanobacterial toxins were purified by high-performance thin-layer chromatography and identified by scanning with a densitometer. The purified components were tested separately by mouse bioassay. Liver damage and nervous disorder were induced by the two peptide and single alkaloid toxins, respectively. |
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