Pulse labelling: a method for measuring microbial growth rates in the ocean |
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| Institution: | 1. Department of Oceanography, Florida State University, Tallahassee, FL32306-3048 USA;2. Department of Marine Sciences, Texas A & M University, Galveston, Texas 77550 USA;3. Institute of Molecular Biophysics, Florida State University, Tallahassee, FL 32303 USA;1. Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, 1206 West Green Street, Urbana, IL 61801, USA;2. Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, 1304 West Green Street, Urbana, IL 61801, USA;3. ATSP Innovations, 60 Hazelwood Drive, Champaign, IL 61820, USA;4. Department of Mechanical and Materials Engineering, University of Denver, 2390 S. York St., Denver, CO 80208, USA;5. Bureau of Reclamation, Denver Federal Center, Denver, CO 80225, USA;6. Frederick Seitz Materials Research Laboratory, University of Illinois, Urbana, IL 61801, USA;7. International Institute for Carbon Neutral Energy Research (WPI-I2CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan;1. Department of Botany, Federal University of Pelotas, C.P. 354, 96160-000, Pelotas, RS, Brazil;2. Department of Animal and Plant Biology, State University of Londrina, 86050-350, Londrina, PR, Brazil;3. Brazilian Agricultural Research Corporation/Embrapa Temperate Climate Agricultural Research Center, BR 392, km 78, 96010-971, Pelotas, RS, Brazil;4. Department of Plant Biology, State University of Campinas, C.P. 6109, 13083-970, Campinas, SP, Brazil;3. Departments of Biochemistry, The Medical College of Wisconsin, Milwaukee, Wisconsin 53226;5. Departments Cell Biology, Neurobiology, and Anatomy, The Medical College of Wisconsin, Milwaukee, Wisconsin 53226;4. Neuroscience Research Center, The Medical College of Wisconsin, Milwaukee, Wisconsin 53226;6. Department of Computational and Physical Sciences, Carroll University, Waukesha, Wisconsin 53186;3. Department of Biology, Georgetown University, Washington, D. C. 20057;4. Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721;5. Division of Neurosurgery, Colket Translational Research, Children''s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104 |
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| Abstract: | Pulse labelling provides a means of generating the growth curve of a microbial population in natural samples. By suitable choice of radioisotopes, a variety of cellular components may be labelled, although adenine to label nucleic acids and 32P to label phospholipids proved most successful. The labelling of phospholipids also enabled us to measure the growth of algae. When cycloheximide was used as a eukaryote inhibitor to prevent grazing it served to reduce the incorporation of adenine into the bacterial component of the population. Sample dilution as means of controlling predation effects actually increased the sample growth rate until the bacterial component reached the same level as in the undiluted sample, at which time the growth rate fell to that found in the undiluted control. This finding suggests that the bacterial population may be maintained at the carrying capacity of the menstruum, and dilution actually induces an artifact rather than eliminate predation effects. |
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