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Cloning of fusaric acid-detoxifying gene from Cladosporium werneckii: a new strategy for the prevention of plant diseases |
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| Institution: | 1. Division of Radiation Effect, Korea Institute of Radiological & Medical Sciences, Seoul 01812, Republic of Korea;2. Health Services Management, KH School of Management, Kyung Hee University, Seoul 02453, Republic of Korea;1. Section for Microbiology, Department of Biology, University of Copenhagen, Universitetsparken 15, Copenhagen, Denmark;2. BioModics ApS, Gregersensvej 7, 2630 Taastrup, Denmark |
| Abstract: | Fusaric acid-detoxifying gene from Cladosporium werneckii was cloned in Escherichia coli. The detoxification of fusaric acid was confirmed chemically by gas chromatography and biologically by using tomato callus cells. The damage caused by fusaric acid was dramatically diminished in tomato cuttings pretreated with E. coli cells containing the cloned plasmid. The findings suggest that genetically engineered microbes could be applicable to the protection of plants from diseases caused by fusaric acid-producing pathogens. |
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