Biosynthesis of lipids by Rhodosporidium toruloides ATCC 10788 |
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| Affiliation: | 1. Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario N6A 5B9, Canada;2. Department of Chemical Engineering, University of Ottawa, Ottawa, Ontario K1N 9B4, Canada;1. Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, South Korea;2. Institute for Ubiquitous Information Technology and App1ications (CBRU), Konkuk University, Seoul, South Korea;1. Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA;2. University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA 15213, USA;3. Department of Human Genetics, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA 15213, USA;1. Laboratory of Biotechnology, School of Chemistry and Food, Federal University of Rio Grande, 96203-900, Rio Grande, RS, Brazil;2. Laboratory of Bioprocess Engineering, School of Chemistry and Food, Federal University of Rio Grande, 96203-900, Rio Grande, RS, Brazil;1. Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan;2. Graduate School of Science, Technology and Innovation (STIN), Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, 657-8501, Japan;3. Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, Cibinong, Bogor, 16991, West Java, Indonesia;1. Department of Food, Environmental and Nutritional Sciences, University of Milan, Italy;2. Eni S.p.A. – Renewable Energy and Environmental R&D Center–Istituto Eni Donegani, Novara, Italy;3. Versalis SPA, Green Chemistry CRNO, Novara, Italy |
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| Abstract: | The limiting amount of nitrogen required to trigger lipid accumulation in the oleaginous yeast Rhodosporidium toruloides ATCC 10788 was studied, batchwise, by subjecting washed mid-exponentially grown cells to nitrogen at levels of 10−2 M down to 10−4 M per g l−1 of lean cells (2–5% fat content) in a mineral medium where glucose was present at 35 g l−1. The results showed that lipid accumulation always started sometime after nitrogen reached a level of 3 × 10−5 M and the specific initial lipid productivity was constant. Furthermore, the cells were subjected to nine combinations of temperature and pH, from (25° C, pH 4.5) to (35° C, pH 7.5) in the mineral medium supplemented with 0.5 g l−1 of yeast extract and 1 g l−1 (NH4)2SO4. As was expected, lipid content in the cells was higher at 25° C, but pH around 6.0–7.5 slightly enhanced the effect of lower temperature. The effect of pH was also noticed to affect the size of changes in the temporal profiles of the oil's fatty acid distribution prior to nitrogen depletion, whereas no significant difference in the fatty acid composition of the oil was shown after exhaustion of nitrogen from the medium for all combinations of temperature and pH. |
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