Detection of cellulolytic actinomycetes using cellulose-azure |
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| Institution: | 1. Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy;2. Dipartimento Scienze della Terra e del Mare, Università degli Studi di Palermo, Via Archirafi 22, 90123 Palermo, Italy;3. Agrichimica Laboratories, Via Sirtori 31, 91025 Marsala, Trapani province, Italy;1. Institute for Micrsensors, -actuators and –systems (IMSAS), University of Bremen, Otto-Hahn-Allee NW1, 28359 Bremen, Germany;2. Microsystems Center Bremen (MCB), Germany;3. microFAB Service GmbH, Bremen, Germany;1. Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan;2. Faculty of Applied Sciences, UCSI University, UCSI Heights, Cheras, Kuala Lumpur 56000, Malaysia;3. Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 32003, Taiwan;1. Dairy Science and Technology Laboratory, Agricultural Research Center of Mabegondo (CIAM), Xunta de Galicia, A Coruña, Spain;2. Food Technology Area, Faculty of Science, University of Vigo, Ourense, Spain;1. Aqua Research Lab, Department of Zoology, University of Delhi, Delhi 110007, India;2. Department of Biotechnology, Delhi Technological University, Delhi 110042, India |
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| Abstract: | A rapid method is described for the detection of cellulolytic, thermophilic actinomycetes involving the use of a dyed cellulose substrate (cellulose-azure) in an agar medium. The test was performed in test tubes in which agar containing cellulose-azure was layered on top of a basal medium (mineral salts agar) resulting in two distinct layers. After inoculation, and incubation at 50°C, strains which were cellulolytic caused release substrates for such a test. |
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