An improved medium for preparation of filterable Escherichia coli hemolysin |
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| Institution: | 1. Department of Endocrinology, Clinical Medical College, Yangzhou University, Yangzhou, China;2. Department of Physiology, Nanjing University of Chinese Medicine Hanlin College, Taizhou, China;3. Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Medical College, Yangzhou University, Yangzhou, China;4. Department of Pathology, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu, China |
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| Abstract: | We found that Tween 80 has potent activity in enhancing passage of E. coli hemolysin through a membrane filter and, on the basis of this finding, we devised a medium, heart infusion broth supplemented with 0.5% Tween 80 and 0.1% glucose (HI-TG) for preparation of culture filtrates containing fairly large amounts of hemolysin. When 27 strains of hemolytic E. coli were cultivated to late logarithmic growth phase in the HI-TG broth at 37°C, the culture filtrates of most strains contained 64 to 128 HU50 (50% hemolytic unit) per ml. From these and other results, we established a routine method for partially purifying E. coli hemolysin. |
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