Long-term preservation of some Rhodospirillaceae by freeze-drying |
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| Affiliation: | 1. Institute of Informatics – Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Porto Alegre, RS, Brazil;2. Department of Industrial Engineering – Universidade Federal do Rio Grande do Sul, Av. Osvaldo Aranha, 99–5° andar, Porto Alegre, RS, Brazil;1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Department of Biomedical Sciences, School of Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USA;2. Department of Anesthesiology, School of Medicine, University of Missouri-Kansas City, Kansas City, MO 64108, USA;1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, USA;2. Cole Eye Institute, Cleveland Clinic, Cleveland, Ohio, USA;3. USC Roski Eye Institute, University of Southern California, Los Angeles, California, USA;4. The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;5. Städtisches Klinikum, Augenklinik, Karlsruhe, Germany;6. Retina Foundation of the Southwest, Dallas, Texas, USA;7. Lions Vision Research and Rehabilitation Center, The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA;8. University Eye Clinic Luebeck at the University Hospital Schleswig-Holstein, Lübeck, Germany;9. Duke University Eye Center, Durham, North Carolina, USA;10. Wills Eye Hospital, Thomas Jefferson University, Philadelphia, Pennsylvania, USA;11. Retina Center of Ohio, Cleveland, Ohio, USA;12. Mayo Clinic, Rochester, Minnesota, USA;13. Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan, USA;14. Retina Consultants of Hawaii, Aiea, Hawaii, USA;15. University of Illinois at Chicago, Chicago, Illinois, USA;p. University of Colorado, Aurora, Colorado, USA;q. University of Minnesota, Minneapolis, Minnesota, USA;r. Eye Clinic Sulzbach, Knappschaft Hospital Saar, Sulzbach, Germany;s. Klinik und Poliklinik für Augenheilkunde, Universitätsklinikum Leipzig, Leipzig, Germany;t. Emory Eye Center, Emory University, Atlanta, Georgia, USA;u. USC Ginsburg Institute for Biomedical Therapeutics, Los Angeles, California, USA |
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| Abstract: | Lyophilization of phototropically grown cultures is difficult as it is essential to maintain strict anaerobic conditions or to avoid the exposure of the cultures to light. During a systematic investigation it was observed that several species of Rhodospirillaceae are able to grow heterotrophically in darkness on organic media and are not damaged by air oxygen under such conditions. Based on this character several oxygenic species of Rhodospirillaceae were grown under heterotrophic conditions and were lyophilized using raffinose (5% w/v) along with skim milk (20% w/v) as a protective agent. More than 30 strains from nine species of Rhodospirillaceae were successfully freeze-dried with this method. All tested cultures proved viable and showed 10–100% survival after lyophilization. During 2–3 years of storage at 9°C no further loss in viability was observed. In such lyophilized cultures no loss in photoautotrophy, diazotrophy or other desirable characters like hydrogen production, pigmentation, etc. was detected. The method is not suited to such anoxygenic Rhodospirillaceae which are not able to grown aerobically in darkness. |
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